Labadie, Paul E.

Agricultural Research Technician

George Kennedy & Michael Reiskind

3312 Gardner Hall

NCSU - Department of Entomology, P.O Box 7613, Raleigh - NC 27695, USA

Position Responsibilities: 
  • Bioinformatics pipelines for population genetics: Unix bash script and command line; Stacks, Samtools, Fastx Toolkit, Bowtie2, BWA, iPyRad, SOAPDenovo.
  • Statistical analysis of population genetics, population structure and parentage. Analysis implementation using PLINK, Genepop, BAPS, Structure, Phylip, GeneClass, Fstat, GDA, Arlequin, GeneLand, R packages (Adegenet, poppr, qqman).
  • Library preparation for NGS Platforms (HiSeq, MiSeq): ddRad-Tags.
  • Perform benchwork duties related to molecular biology and general biology research.
  • DNA extractions (ethanol, chelex, column membrane, agar excision) and purification (DNA clean-up kits, phenol-chlorophorm, AMPure-Xp beads)
  • PCR and PCR troubleshooting
  • Genetic Profiling using STRs, RFLPs, SNPs
  • Setup, servicing and operation of Li-Cor 4300 Sequencer and Li-Cor 4200
  • Genomic Sequencing data production (Sanger data and NextGenSeq data) and analysis using Vector NTI, MEGA, BioEdit
  • Microsatellites design and optimization using MsatCommander, Primer3
  • High Throughput Sequencing and Data Analysis of genetic data for the agro-science industry (Dow, Bayer, Syngenta, BASF)
  • Pesticides efficacy trials: laboratory experiments and field bioassays
  • Stock solutions preparation and titration
  • Field collections and laboratory rearing of termites, ants and other insects
  • Supervision and coordination of laboratory personnel
  • TA for ENT591/791course: "Techniques in Molecular Ecology"
  • Lab supplies and chemicals inventory managment
  • Web Design
  • IT tech support (Microsoft and Linux)


 Burford Reiskind, M. O., Coyle, K., Daniels, H. V., Labadie, P., Reiskind, M. H., Roberts, N. B., Roberts, R. B., Schaff, J. and Vargo, E. L. (2016), Development of a universal double-digest RAD sequencing approach for a group of nonmodel, ecologically and economically important insect and fish taxa. Mol Ecol Resour, 16: 1303–1314. doi:10.1111/1755-0998.12527. PDF

Haifig, Ives., Vargo, Edward L., Labadie, Paul., Costa-Leonardo, Ana Maria. 2016. Unrelated secondary reproductives in the neotropical termite Silvestritermes euamignathus (Isoptera: Termitidae). The Science of Nature, 9(103)-1: 1432-1904. doi:10.1007/s00114-015-1325-0. PDF

Perdereau, E., Bagnères, A.-G., Vargo, E.L., Baudouin, G., Xu, Y., Labadie, P., Dupont, S. and Dedeine, F. (2015), Relationship between invasion success and colony breeding structure in a subterranean termite. Mol Ecol, 24: 2125–2142. doi:10.1111/mec.13094. PDF

Vargo, E. L., P. E. Labadie and K. Matsuura. 2012. Asexual queen succession in the subterranean termite Reticulitermes virginicus. Proceedings of the Royal Society of London, B 279: 813-819. PDF

Brightwell, R.J., Labadie, P. E. and Silverman, J. 2010. Northward expansion of the invasive Argentine ant, Linepithema humile, (Hymenoptera: Formicidae) is constrained by winter soil temperatures. Environmental Entomology 39(5):1659-1665. 2010. PDF

Matsuura, K., E. L. Vargo, K. Kawatsu, P. E. Labadie, H. Nakano, T. Yashiro and K. Tsuji. 2009. Queen succession through asexual reproduction in termites. Science 27 323:1687. PDF