Labadie, Paul E.

Agricultural Research Technician

Supervisor: 
George Kennedy & Michael Reiskind
Office: 

3312 Gardner Hall

NCSU - Department of Entomology, P.O Box 7613, Raleigh - NC 27695, USA

Phone: 
919.515.1662
Fax: 
919.515.7746
Position Responsibilities: 
  • Bioinformatics pipelines for population genetics: Unix bash script and command line; Stacks, Samtools, Fastx Toolkit, Bowtie2, BWA, iPyRad, SOAPDenovo.
  • Statistical analysis of population genetics, population structure and parentage. Analysis implementation using PLINK, Genepop, BAPS, Structure, Phylip, GeneClass, Fstat, GDA, Arlequin, GeneLand, R packages (Adegenet, poppr, qqman).
  • Library preparation for NGS Platforms (HiSeq, MiSeq): ddRad-Tags.
  • Perform benchwork duties related to molecular biology and general biology research.
  • DNA extractions (ethanol, chelex, column membrane, agar excision) and purification (DNA clean-up kits, phenol-chlorophorm, AMPure-Xp beads)
  • PCR and PCR troubleshooting
  • Genetic Profiling using STRs, RFLPs, SNPs
  • Setup, servicing and operation of Li-Cor 4300 Sequencer and Li-Cor 4200
  • Genomic Sequencing data production (Sanger data and NextGenSeq data) and analysis using Vector NTI, MEGA, BioEdit
  • Microsatellites design and optimization using MsatCommander, Primer3
  • High Throughput Sequencing and Data Analysis of genetic data for the agro-science industry (Dow, Bayer, Syngenta, BASF)
  • Pesticides efficacy trials: laboratory experiments and field bioassays
  • Stock solutions preparation and titration
  • Field collections and laboratory rearing of termites, ants and other insects
  • Supervision and coordination of laboratory personnel
  • TA for ENT591/791course: "Techniques in Molecular Ecology"
  • Lab supplies and chemicals inventory managment
  • Web Design
  • IT tech support (Microsoft and Linux)

Publications:

 Burford Reiskind, M. O., Coyle, K., Daniels, H. V., Labadie, P., Reiskind, M. H., Roberts, N. B., Roberts, R. B., Schaff, J. and Vargo, E. L. (2016), Development of a universal double-digest RAD sequencing approach for a group of nonmodel, ecologically and economically important insect and fish taxa. Mol Ecol Resour, 16: 1303–1314. doi:10.1111/1755-0998.12527. PDF

Haifig, Ives., Vargo, Edward L., Labadie, Paul., Costa-Leonardo, Ana Maria. 2016. Unrelated secondary reproductives in the neotropical termite Silvestritermes euamignathus (Isoptera: Termitidae). The Science of Nature, 9(103)-1: 1432-1904. doi:10.1007/s00114-015-1325-0. PDF

Perdereau, E., Bagnères, A.-G., Vargo, E.L., Baudouin, G., Xu, Y., Labadie, P., Dupont, S. and Dedeine, F. (2015), Relationship between invasion success and colony breeding structure in a subterranean termite. Mol Ecol, 24: 2125–2142. doi:10.1111/mec.13094. PDF

Vargo, E. L., P. E. Labadie and K. Matsuura. 2012. Asexual queen succession in the subterranean termite Reticulitermes virginicus. Proceedings of the Royal Society of London, B 279: 813-819. PDF

Brightwell, R.J., Labadie, P. E. and Silverman, J. 2010. Northward expansion of the invasive Argentine ant, Linepithema humile, (Hymenoptera: Formicidae) is constrained by winter soil temperatures. Environmental Entomology 39(5):1659-1665. 2010. PDF

Matsuura, K., E. L. Vargo, K. Kawatsu, P. E. Labadie, H. Nakano, T. Yashiro and K. Tsuji. 2009. Queen succession through asexual reproduction in termites. Science 27 323:1687. PDF